TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Descriptiona	Reference or source
Strains
A. veronii
HM21R	Spontaneous Rfr mutant of wild-type HM21	16
HM21RS	Spontaneous Smr mutant of HM21R	46
JG736	HM21R lpp-1 (lpp::mTn5Km2STM); Rfr Kmr	This study
E. coli
TOP10	Cloning strain; Smr	Invitrogen
S17-1λpir	Strain used for conjugation with A. veronii	14
DH5αλpir	Cloning strain capable of maintaining suicide vectors	5
CC118λpir	Carrying helper plasmid pEVS104	57
Plasmids
pUTminiTn5Km2	Contains Tn used for mutant generation; Kmr	19
pEVS104	Helper plasmid; tra trb Kmr	57
pCR2.1	Bacterial cloning vector; Kmr Apr	Invitrogen
pAS10	Derivative of pCR2.1 containing lpp; Kmr Apr	This study
pMMB207	Low-copy-number broad-host-range vector; Cmr	36
pAS11	Derivative of pMMB207 containing lpp; Cmr	This study

^aRf^r, rifampin resistance; Km^r, kanamycin resistance; Ap^r, ampicillin resistance; Cm^r, chloramphenicol resistance; Sm^r, streptomycin resistance.