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. 2007 Jul 6;189(19):6763–6772. doi: 10.1128/JB.00685-07

TABLE 2.

Characterization of mutants

Predicted function and strain(s) Disrupted gene or gene product GenBank accession no. Identity, similarity,a e value Symbiosisb Growth in bloodc
Surface modification
    JG535 Glycosyltransferase YP_727333 49, 68, e−86 0.0099***/*** 1.27
    JG736 Outer membrane lipoprotein YP_926710 56, 72, e−11 0.0031***/** 0.60
    JG738 Methyltransferase type 11 YP_944741 24, 49, e−9 0.018***/*** 1.26
Regulatory
    JG574 rRNA operon X74684 99, 99, e0 0.15**/* 0.61
    JG697 GTP-binding protein YP_857656 97, 100, e−70 0.092***/— 0.06
    JG730 Response regulator YP_855452 77, 85, e−81 0.00020***/** 2.44
    JG741 Exoribonuclease II YP_855087 95, 98, e−76 0.28*/* 0.76
Nutritional
    JG537 Phosphate ABC transporter YP_270310 92, 96, e−10 0.033***/* 0.80
    JG698 GufA protein YP_856244 95, 97, e−38 0.23*/— 0.01
    JG750 Threonine/serine transporter NP_755741 85, 94, e−168 0.10*/** 1.34
Host interaction
    JG752 ascU CAD56767 100, 100, e−40 0.13***/*** 0.73
Unknown
    JG521 Hypothetical protein YP_737079 33, 54, e−19 0.10**/* 1.19
    JG523 No similarity detected 0.32*/* 1.07
    JG532 Conserved hypothetical protein ZP_01669898 53, 69, e−38 0.63**/* 0.36
    JG533 KAP family P-loop domain protein YP_856709 71, 80, e−80 0.33**/— 0.60
    JG538 Peptidase S15 ZP_01542412 66, 78, e−59 0.43*/** 1.87
    JG573 Hypothetical protein NP_930352 52, 70, e−70 0.0090***/* 2.07
    JG735 Hypothetical protein XP_001153743 27, 34, e−5 0.000076***/** 0.95
    JG751 Hypothetical protein YP_856595 84, 88, e−95 0.33*/* 0.92
    JG753 No similarity detected 0.19**/*** 1.46
Wild type
    HM21R 1.3 1.07
a

Percent identity and percent similarity by a BLASTX or BLASTN search.

b

Mean in vivo CI values. CI values were calculated by dividing the mutant/competitor strain output ratio by the input ratio of the mutant to the competitor. The first set of asterisks after each value represents the results of a two-tailed, one-sided t test that was used to determine whether the CI differed from 1, while the second set of asterisks represents the results of an unpaired two-tailed, two-sided t test that was used to determine whether the in vivo CI differed from the in vitro CI. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; —, not significantly different.

c

Mean in vitro CI values.