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Kinetic values for substrate hydrolysis by AtzB
| Kinetic measure | Value for type of reaction on substrate (mean ± SD)
|
||||
|---|---|---|---|---|---|
| Deamination
|
Dechlorination
|
||||
| IEOT | IMOT | IIOT | CIOT | CEOT | |
| Km (μM) | 20 ± 2 | 40 ± 2 | 29 ± 3 | 120 ± 8 | 230 ± 20 |
| kcat (s−1) | 3.2 ± 0.2 | 1.4 ± 0.1 | 0.8 ± 0.1 | 1.9 ± 0.1 | 1.6 ± 0.1 |
| kcat/Km (mM−1 s−1) | 160 ± 20 | 35 ± 2 | 28 ± 6 | 16 ± 1 | 7 ± 1 |
