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. 2007 Jul 27;189(19):6901–6912. doi: 10.1128/JB.00763-07

FIG. 10.

FIG. 10.

A neighbor-joining tree for the ATPase components of the glycine betaine and choline ABC transporters that have been functionally characterized in prokaryotes and those examined in this study. The osmoregulated E. coli YehX protein was included for comparison, although it is not functional (12). The numbers in parentheses indicate the number of genes in the locus encoding each transporter. The proteins that are shown include AAO56549 (P. syringae pv. tomato PSPTO_3060), AAC75724 (E. coli ProV), AAL21694 (Salmonella enterica serovar Typhimurium ProV), AAQ06630 (E. chrysanthemi OusBV), P4692 (B. subtilis OpuAA), CAC99092 (Listeria monocytogenes GbuA), AAF37878 (L. lactis OpuAA), AAO58021 (P. syringae pv. tomato PSPTO_4575), AAA60492 (E. coli YehX), Q45460 (B. subtilis OpuBA), O34992 (B. subtilis OpuCA), CAC99506 (L. monocytogenes OpuCA), CAC46836 (S. meliloti Prb), CAC48813 (S. meliloti EhuA, which transports ectoine and glycine betaine [28]), AAO54006 (P. syringae pv. tomato PSPTO_0462), CAC46980 (S. meliloti ChoV), AAO58699 (P. syringae pv. tomato PSPTO_5273), and CAC47281 (S. meliloti HisV, which transports histidine and glycine betaine [8]). Sequences were aligned using ClustalW (13), and a guide tree was constructed by the neighbor-joining method.