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Kinetics of OpuC-mediated uptake of glycine betaine (A) and choline (B) in E. coli MKH13(pMEopuC). Log-phase cells grown in M63 medium amended with 0.5 M of NaCl were suspended in 50 mM of phosphate buffer (pH 7) containing 0.5 M of NaCl, 0.2% glucose, and [14C]glycine betaine or [14C]choline at various concentrations. The results are means ± SEM (n = 3).
