TABLE 1.
Plasmids and strains used in this study
| Strain or plasmid | Description/relevant genotype | Reference or source |
|---|---|---|
| Escherichia coli | ||
| DH5α | Host for cloning | Invitrogen |
| MG1655 | Host for mutagenesis | 15 |
| MKH13 | ΔputPA101 ΔproP2 ΔproU::spc-608 ΔbetTIBA; Spr | 31 |
| Pseudomonas syringae pv. tomato | ||
| DC3000 | Wild type; Rfr | 41 |
| 0462::pKO | DC3000 with PSPTO_0462::pKnockout-Ω; Rfr Spr | This work |
| 3060::pKO | DC3000 with PSPTO_3060::pKnockout-Ω; Rfr Spr | This work |
| 4575::pKO | DC3000 with PSPTO_4575::pKnockout-Ω; Rfr Spr | This work |
| 5273::pKO | DC3000 with PSPTO_5273::pKnockout-Ω; Rfr Spr | This work |
| DC3000ΔopuCA | DC3000 with ΔPSPTO_4575; Rfr | This work |
| Plasmids | ||
| pKnockout-Ω | Suicide vector for rapid gene inactivation in P. syringae; Smr Spr | 55 |
| pKD13 | Template for kan cassette flanked by FLP recombination target sites; Apr Kmr | 15 |
| pKD46 | Encodes λ Red recombinase; repA101ts; Apr | 15 |
| pFlp2 | Encodes Flp recombinase, suicide vector in P. syringae; Apr | 26 |
| pRK2013 | RP4 transfer functions for mobilization; Kmr | 17 |
| pRK2073 | pRK2013 with Tn7 in the Kmr gene; Smr Spr | 6 |
| pKO4575-8 | pKnockout-Ω containing 9,018-bp PCR-amplified fragment with PSPTO_4575-PSPTO_4578 | This work |
| pME6041 | E. coli-Pseudomonas shuttle and cloning vector; oriVpVSIoriVp15AoriT; Kmr | 24 |
| pMEopuC | pME6041 containing a 9.6-kb genomic fragment with PSPTO_4575-PSPTO_4578 | This work |