TABLE 3.
Organism | ATPase | Fnb | Result for indicated regiona
|
|||
---|---|---|---|---|---|---|
CBSc | CBS1d | CBS2d | C-terminal taild | |||
E. coli K-12 | ProV | + | 4 × 10−5 | + (6:10, 56) | − (7:2, 52) | 0 (1:1, 7) |
S. enterica serovar Typhimurium LT2 | ProV | + | 5 × 10−9 | + (7:10, 59) | − (7:2, 52) | 0 (1:1, 7) |
E. chrysanthemi | OusBV | + | 3 × 10−11 | + (7:13, 59) | − (5:1, 52) | − (2:1, 7) |
L. lactis | OpuAA | + | 1 × 10−13 | + (6:9, 56) | − (7:4, 52) | − (11:2, 17) |
L. monocytogenes | GbuA | + | 5 × 10−18 | + (7:11, 56) | − (6:4, 52) | − (1:0, 8) |
B. subtilis | OpuAA | + | 5 × 10−20 | + (6:11, 57) | − (8:4, 54) | − (5:1, 24) |
B. subtilis | OpuCA | + | 5 × 10−25 | + (7:10, 55) | + (5:8, 53) | − (4:0, 14) |
B. subtilis | OpuBA | + | 2 × 10−25 | + (7:10, 54) | + (8:9, 53) | − (3:1, 14) |
L. monocytogenes | OpuCA | + | 3 × 10−26 | − (8:7, 55) | + (7:10, 53) | − (10:1, 31) |
P. syringae DC3000 | PSPTO_4575 | + | 4 × 10−16 | − (12:7, 53) | − (8:4, 53) | + (1:3, 20) |
P. syringae B728a | Psyr4249 | + | 4 × 10−16 | − (12:7, 53) | − (8:4, 53) | + (2:3, 23) |
P. aeruginosa PAO1 | PA3891 | + | 1 × 10−19 | − (10:9, 53) | − (7:3, 53) | + (1:3, 20) |
E. coli MC4100 | YehX | − | 0.42 | None | None | None |
L. monocytogenes | Lmo1421 | − | 0.41 | None | None | None |
S. meliloti 1021 | ChoV | − | 0.14 | None | None | None |
S. meliloti 1021 | EhuA | − | >1.0 | None | None | None |
S. meliloti 1021 | PrbV | − | >1.0 | None | None | None |
S. meliloti 5000 | HisV/HutV | − | >1.0 | None | None | None |
P. syringae DC3000 | PSPTO_5273 | − | >1.0 | None | None | None |
P. syringae DC3000 | PSPTO_0462 | − | >1.0 | None | None | None |
P. syringae DC3000 | PSPTO_3060 | − | 0.49 | None | None | None |
Values in parentheses are ratios of the anionic residue number/cationic residue number and the total numbers of amino acids in the indicated domain or tail.
The ability (+) or inability (−) to mediate the uptake of osmoprotectant compounds in response to increasing osmolality is indicated. Functional information was obtained from E. coli ProV (21), S. enterica serovar Typhimurium ProV (49), OusBV (14); L. lactis OpuAA (52); GbuA (3); B. subtilis OpuAA (31); B. subtilis OpuCA (30); OpuBA (30); L. monocytogenes OpuCA (4, 54); PSPTO_4575, Psyr4249, PA3891, and PP0868 (this study); YehX (lack of uptake in MKH13, which contains an intact yehX); Lmo1421 (3, 53); ChoV (19); EhuA (28); PrbV (1); HisV/HutV (8); and PSPTO_5273, PSPTO_0462, and PSPTO_3060 (this study).
E values for the tandem CBS domains were obtained by using the Pfam HMM database (http://pfam.janelia.org/hmmsearch.shtml).
The predicted net charge of the CBS1 domain, CBS2 domain, or C-terminal tail based on the number of cationic or anionic residues, with + indicating more cationic residues, − indicating more anionic residues, and 0 indicating equal numbers.