Figure 1.

Southern blot hybridization of PCR products amplified from woolly monkey (lanes WM) serum or HBV containing serum. DNA was extracted from 1:10 dilutions of woolly monkey or HBV containing serum. U, undiluted; −1 to −5 = 10⁻¹ to 10⁻⁵ dilutions. PCR was conducted with primers to the core gene. Hybridization of identical membranes was performed with an HBV DNA probe under high (A) or low (B) stringency conditions or with the isolated DNA fragment from amplification of the woolly monkey serum (C) under low-stringency conditions.