TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source or reference |
|---|---|---|
| OI1085 | Che+trpF7 hisH2 metC133 | 35 |
| OI3135 | ΔcheC1 | 25 |
| OI3165 | ΔcheC1 amyE5720::cheC3 | 25 |
| OI4218 | OI1085 amyE5720::B. halodurans cheX1 | This work |
| OI4219 | ΔcheC1 amyE5720::B. halodurans cheX1 | This work |
| OI4222 | ΔcheC1 amyE5720::B. halodurans cheX2 (cheX-S87A) | This work |
| OI4223 | ΔcheC1 amyE5720::B. halodurans cheX3 (cheX-E91A) | This work |
| OI4224 | ΔcheC1 amyE5720::B. halodurans cheX4 (cheX-N94A) | This work |
| OI4225 | ΔcheC1 amyE5720::B. halodurans cheX5 (cheX-P116A) | This work |
| OI4233 | ΔcheC1 amyE5720::B. halodurans cheX6 (cheC RBS) | This work |
| BL21 | E. coli protease-deficient expression host | Amersham |
| TG1 | E. coli cloning host | Amersham |
| pBluescriptSK- | Cloning vector, Ampr | Stratagene |
| pGEX-6P-2 | GST tag expression vector, Ampr | Amersham |
| pDR67 | amyE integration plasmid, pSpac, Ampr, Cmr | 13 |
| pHS102 | pGEX-6P-2::cheY1 | 30 |
| pTM0 | pGEX-6P-2::fliY5 | This work |
| pTM18 | pGEX-6P-2::cheC2 | 31 |
| pTM75 | pGEX-6P-2::B. halodurans cheX7 | This work |
| pTM74 | pBluescriptSK-::B. halodurans cheX1 | This work |
| pTM49 | pBluescriptSK-::B. halodurans cheX6 | This work |
| pTM53 | pDR67::B. halodurans cheX1 (opRBS) | This work |
| pTM50 | pDR67::B. halodurans cheX6 (cheC RBS) | This work |