TABLE 1.
MukB content of whole-cell extracts and isolated nucleoids
| Straina | MukB content (pmol/mg) in the indicated fractionb
|
|
|---|---|---|
| Cell extract | Nucleoidc | |
| DH5α (pBAD) | 7.7 ± 1.7 | 28 ± 12 (>90) |
| DH5α (pBEF++) | 440 | 320 (85) |
| DH5α (pEF++) | 10 ± 5 | 2.4 ± 0.8 (30 ± 20) |
| MG1655 (pEF) | 9 | 9 (93) |
| MG1655 (pBAD) | 12 ± 1 | 42 ± 10 (86 ± 2) |
| OU102 (pBAD) | 8 ± 5 | 4.0 ± 0.6 (57 ± 19) |
| MG1655 (pSmtA++) | 5 | 8 (96) |
| MG1655 (pTopA++) | 6 | 11 (71) |
| MG1655 (pB337++) | 8 | 13 (71) |
a
++, plasmid designed to overproduce the indicated protein or fragment was induced with arabinose.
b
The data are normalized to the total protein in a given fraction. When standard deviations are shown, the data are the average of two independent experiments.
c
The percentage of the nuceloid-associated MukB that was released by DNase I treatment is given in parentheses.