TABLE 2.
MukF and MukE content of whole-cell extracts and isolated nucleoids
| Straina | MukF content in the indicated fraction (pmol/mg)b
|
MukE content in the indicated fraction (pmol/mg)b
|
||
|---|---|---|---|---|
| Cell extract | Nucleoid | Cell extract | Nucleoid | |
| DH5α (pBAD) | ND | 2.5 | 1.7 ± 0.7 | 9.6 ± 2.2 (95 ± 2) |
| DH5α (pBEF++) | 450 | 400 (30) | 370 | 40 (64) |
| DH5α (pEF++) | >210 | 40 (16) | >180 | 100 (40) |
| MG1655 (pBAD) | ND | ND | 3.4 | 14 |
| GC7528 (pBAD) | 1.6 | <0.2 | 1.1 | 0.2 |
a
++, plasmid designed to overproduce the indicated protein was induced with arabinose.
b
The percentage of the nucleoid-associated proteins that was solubilized by DNase I treatment is given in parentheses. ND, not detrmined.