TABLE 1.
Identification of CBPs located in OM fraction of F. succinogenes S85
| Band | FSU no.a | Protein name or description | No. matched (%)b | Coverage (%) | Mw_t/Mw_ec | pI_t/pI_t_ce | Feature |
|---|---|---|---|---|---|---|---|
| 1 | 809 | GH family 9 | 5/10 (50) | 7.0 | 233.0/250 | 4.97/9.63 | Present in all strains |
| 2 | 2502 | Fibro-slime domain protein | 46.0d | 169.4/180.0 | 5.00/10.17 | Present in all strains | |
| 3 | 2398 | TPR domain protein | 7/9 (77) | 12.0 | 146.7/150 | 8.5/10.00 | Only present in S85 grown on cellulose |
| 4 | 382 | Cellulose-binding protein, CelF | 5/16 (31) | 8.0 | 118.0/120 | 7.81/9.8 | Absent from Ad4 |
| 5 | 718 | Membrane protein | 6/18 (33) | 11.0 | 92.4/92 | 9.01/10.01 | Present in all strains |
| 6 | 1516 | AAA family protein | 7/18 (38) | 11.0 | 89.5/90 | 6.43/6.77 | Present only in S85 grown on cellulose |
| 7 | 2397 | TPR domain protein | 10/26 (38) | 25.0 | 83.8/80 | 5.5/5.1 | Absent from Ad4 |
| 8 | 2396 | OmpA protein | 7/10 (70) | 19.0 | 53.0/55 | 4.86/10.15 | Present only in S85 grown on cellulose |
| 9 | 2012 | GH family 18 | 7/16 (43) | 34.0 | 37.5/37 | 4.4/8.05 | Absent from Ad4 |
| 10 | 1231 | Hypothetical protein | 5/11 (45) | 18.0 | 31.5/27 | 7.60/5.35 | Present only in S85 grown on cellulose |
| 11 | 2007 | Cellulose binding protein | 8/21 (38) | 32.0 | 29.2/27 | 6.46/8.26 | Absent from Ad4 |
| 12 | 2567 | Type IV pilin protein | 62.0d | 17.5/18 | 5.43/4.23 | Absent from mutants | |
| 13 | 794 | Conserved hypothetical protein | 4/8 (50) | 33.0 | 16.1/17 | 7.64/5.72 | Absent from mutants |
a
F. succinogenes gene number.
b
No. of peptides matched/total (%).
c
Mw_t and Mw_e indicate theoretical and experimental molecular masses, respectively.
d
Coverage by tandem mass spectrometry.
e
pI_t and pI_t_c indicate theoretical isoelectric points of the proteins and their C-terminal 60 amino acids, respectively.