TABLE 2.
List of OM proteins of S85 identified by 2-DE and MALDI-TOF mass spectrometrya
| Protein no. | FSU no. | Protein name | Presence of protein inb:
|
Mw_t | Mw_e | pI_t | pI_e | Matched (%)c | % Cov. | PSort predictiond | SignalP predictiond | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| S85 G | AD1 G | AD4 G | S85 C | |||||||||||
| 1 | 864 | Lipoprotein | N | N | N | Y | 46.3 | 50-60 | 4.6 | 4.6 | 5/15 (33) | 12.0 | OM | Y |
| 2 | 700 | Outer membrane efflux protein precursor | Y | N | N | Y | 52.3 | 50 | 5.3 | 5.1 | 6/26 (23) | 17.0 | OM | Y |
| 3 | 222 | GGDEF domain protein | N | N | N | Y | 49.8 | 50 | 5.9 | 6.0 | 5/20 (25) | 14.0 | CP | N |
| 4 | 2401 | Hypothetical protein | N | N | N | Y | 28.2 | 30 | 4.6 | 5.0 | 8/28 (28) | 45.0 | OM | Y |
| 5 | 2078 | OmpA family protein | Y | Y | Y | Y | 72.1 | 34 | 4.7 | 4.7 | 10/22 (45) | 28.0 | OM | Y |
| 6 | 2096 | Lipoprotein, | Y | Y | Y | Y | 72.5 | 34 | 4.5 | 4.7 | 9/30 (30) | 21.0 | OM | Y |
| 7 | 2388 | Transcriptional regulator, LysR family | Y | Y | Y | Y | 34.2 | 35 | 7.6 | 7.0 | 5/31 (16) | 24.0 | CP | N |
| 8 | 474 | Hypothetical protein | N | N | N | Y | 30.6 | 32 | 5.2 | 6.0 | 10/57 (17) | 50.0 | IM | Y |
| 9 | 2016 | P3 domain protein | N | N | N | Y | 31.0 | 30 | 7.1 | 7.2 | 7/29 (24) | 27.0 | IM | Y |
| 10 | 2450 | Lipoprotein, putative | Y | Y | Y | Y | 26.0 | 25 | 5.2 | 5.5 | 6/43 (13) | 29.0 | OM | Y |
| 11 | 2007 | Cellulose-binding protein | Y | Y | Y | Y | 29.2 | 25 | 6.5 | 6.0 | 10/46 (21) | 51.0 | IM | Y |
| 12 | 230 | Hypothetical protein | Y | Y | Y | Y | 43.0 | 43 | 4.6 | 4.6 | 13/38 (34) | 45.0 | CP | Y |
| 13 | 2404 | Hypothetical protein | Y | Y | Y | Y | 46.1 | 42 | 4.7 | 4.7 | 10/35 (28) | 27.0 | OM | Y |
| 14 | 1029 | Hypothetical protein | Y | Y | Y | Y | 42.2 | 43 | 4.8 | 5.0 | 20/42 (47) | 76.0 | OM | Y |
| 15 | 2078 | OmpA family protein | Y | Y | Y | Y | 72.1 | 74 | 4.7 | 4.7 | 11/24 (45) | 26.0 | OM | Y |
| 16 | 2009 | Hypothetical protein | Y | Y | N | Y | 76.4 | 74 | 5.0 | 5.2 | 5/21 (23) | 16.0 | OM | Y |
| 17 | 2010 | Hypothetical protein | Y | Y | N | Y | 75.5 | 74 | 5.5 | 5.5 | 7/12 (58) | 23.0 | OM | Y |
| 18 | 2285 | conserved hypothetical protein | Y | Y | Y | Y | 77.1 | 70 | 5.7 | 5.7 | 15/53 (28) | 41.0 | CP | N |
| 19 | 2567 | Type IV pilin | Y | N | N | Y | 17.5 | 23 | 5.4 | 5.0 | 9/24 (37) | 85.0 | OM | Y |
| 20 | 609 | Lipoprotein, putative | Y | Y | Y | Y | 18.2 | 22 | 4.9 | 5.0 | 8/26 (30) | 49.0 | OM | Y |
| 21 | 2807 | Hypothetical protein | Y | Y | Y | Y | 36.4 | 35 | 7.1 | 7.3 | 5/9 (55) | 27.0 | OM | Y |
| 22 | 2581 | Hypothetical protein | Y | Y | Y | Y | 27.1 | 40 | 5.9 | 6.0 | 4/24 (16) | 22.0 | OM | Y |
| 23 | 257 | Endo-1,4-β-xylanase z (ClCBase; Cel10A) | Y | N | N | Y | 69.8 | 65 | 9.0 | 6.5 | 9/16 (56) | 20.0 | OM | Y |
| 24 | 3156 | Conserved hypothetical protein | Y | Y | Y | Y | 39.6 | 37 | 5.2 | 5.0 | 5/25 (20) | 24.0 | CP | N |
| 25 | 1783 | Hypothetical protein | N | Y | N | Y | 17.9 | 20 | 7.9 | 8.0 | 6/22 (27) | 55.0 | OM | Y |
Abbreviations: G, OM samples prepared from Glucose-grown cells; C, OM samples prepared from cellulose-grown cells; Y, present in the sample; N, not detected in the sample; IM, inner membrane; CP, cytoplasm; FSU, Fibrobacter succinogenes (see footnote a of Table 1); Mw_t and Mw_e, theoretical and experimental molecular masses, respectively; p_t and pI_e, theoretical and experimental isoelectric points of the protein, respectively; Y/N, the signal peptide is present/absent; Cov., coverage.
Underling indicates that the protein was induced in the presence of cellulose.
No. of peptides matched/total (%).
Predicted by SignalP, TatP, and LipoP programs at http://www.cbs.dtu.dk/services/.