FIG. 7.

Partial time-dependent surface proteolysis of M. genitalium WT strain. Intact cells were treated with proteinase K, and reactions were stopped by adding 1 mM PMSF after 5, 10, 15, 30, 45, and 60 min. Protein profiles from cells that underwent proteolysis were analyzed by Western blotting using anti-P140 (A) and the HMW1 antiserum (B). C1 corresponds to the protein profile of untreated cells, and C2 corresponds to the protein profile resulting when PMSF was added to the cells prior to proteinase K. M, apparent molecular mass prestained marker (Invitrogen).