TABLE 1.
Primers
| Primer | Sequence (5′-3′)a |
|---|---|
| 5′BEKOMG312 | GGTACCCCTGCTTTGTGAATCCATGG |
| 3′BEKOMG312 | GAATTCAGTAATGGATTCAATGAGTGC |
| 5′BDKOMG312 | GGATCCAGGGTCTTCTTCAACAGTGG |
| 3′BDKOMG312 | TCTAGATAGTGTTTTATTAGCACTAACC |
| 5′MG312 | GTCGACTAGTATTTAGAATTAATAAAGTATGGCTAAAAACAAGCAATCG |
| 3′MG312 | CTGCAGTTAATAATCAAGGCTAAAATCAC |
| 5′MG312ΔN-t | GTCGACTAGTATTTAGAATTAATAAAGTATGGAATCTCAACCAACTGATG |
| 3′MG312ΔC-t | CTGCAGTTAAAGATCTGATTTTTTATTGAAATTATC |
| 3′MG312ΔCD | AGATCTGATTTAGTGGAGATCCACTGGTC |
| 5′MG312ΔEAGR | AAGCAAGAAGAGAATGATTCCACTAAAGAATCTCAAC |
| 3′MG312ΔEAGR | GTTGAGATTCTTTAGTGGAATCATTCTCTTCTTGCTT |
| 3′MG312EcoRI | GAATTCAGAATTTAATGAATCATTG |
a
The restriction sites introduced in the 5′ ends of the primers are underlined; the 22 nucleotides from the putative MG_438 gene promoter region (26) are in italics; the ATG and stop codons introduced into two primers are in boldface.