TABLE 2.
Summary of phenotypes of the ΔMG_312 and ΔMG_312 derivative mutants
| Strain genotype | Protein levela
|
HA phenotypec | Cell morphology | ||||||
|---|---|---|---|---|---|---|---|---|---|
| MG312b | MG386 | MG218 | P140 | P110 | MG317 | MG217 | |||
| WT | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | Flask shape |
| ΔMG_312 | − | ++ | ++ | + | + | ± | ± | + | Rounded/pleomorphic |
| ΔMG_312 + MG_312 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | Near WT |
| ΔMG_312 + MG_312ΔRep | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | Near WT |
| ΔMG_312 + MG_312ΔEAGR | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | Near WT |
| ΔMG_312 + MG_312ΔN-t | ++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | Near WT/elongated |
a
Relative amounts of protein: −, none; ±, very reduced; +, significantly reduced; ++, slightly reduced; +++, WT level.
b
For the MG_312 derivative mutants, symbols indicate the stability of the MG312 derivative form.
c
Qualitative HA activity: +, intermediate HA activity; +++, WT HA activity.