TABLE 2.
Mass values of intact flagellin and N136-K255 peptide fragments from P. syringae pv. glycinea race 4
| Flagellin or peptide fragmenta | [M + H]+
|
Δ[M + H]+ (observed − calculated) | |
|---|---|---|---|
| Observedb | Calculatedc | ||
| Intact flagellin (A2-Q282) | |||
| Wild type | 32,380, 32,515, 32,668 | 29,148 | 3,232, 3,367, 3,520 |
| Δorf1 | 29,154 | 29,148 | 6 |
| Peptide fragment (N136-K255) | |||
| WT | 15,296, 15,444, 15,591 | 12,074 | 3,222, 3,370, 3,517 |
| S143A | 14,742, 14,890, 15,036 | 12,058 | 2,684, 2,832, 2,978 |
| S164A | 14,746, 14,894, 15,040 | 12,058 | 2,688, 2,836, 2,982 |
| S176A | 14,748, 14,893, 15,038 | 12,058 | 2,690, 2,835, 2,980 |
| S183A | 14,747, 14,894, 15,044 | 12,058 | 2,689, 2,836, 2,986 |
| S193A | 14,751, 14,899, 15,045 | 12,058 | 2,693, 2,841, 2,987 |
| S201A | 14,747, 14,894, 15,038 | 12,058 | 2,689, 2,836, 2,980 |
| 6 S/A | 11,976 | 11,978 | −2 |
| Δorf1 | 12,072 | 12,074 | −2 |
| Δorf2 | 13,292-14,858 | 12,074 | 1,218-2,784 |
| Δorf3 | 15,298, 15,446, 15,591 | 12,074 | 3,224, 3,372, 3,517 |
a
The N-terminal methionine of intact flagellin is subject to posttranslational cleavage (data not shown). For the Δorf2 peptide fragment, peaks were observed as broad peaks by heterogeneity.
b
Major peaks in MALDI-TOF mass spectra.
c
Calculated value by deduced amino acid sequence.