TABLE 3.

Assignment of NMR signals^a of glycan on S201 of the D200-A211 peptide

Residuea	Position	1H NMR			13C NMR δ (ppm)
		δ (ppm)	Multiplicityb (W1/2, Hz)	J (Hz)
Ser201	Amide carbonyl				172.5c
	α	4.599	dd	4.3, 3.7	55.53
	β	4.055	dd	10.0, 4.3	67.67
	3.732	dd	10.0, 3.7
α-l-Rhap 1	1	4.888	br s (4.5)		99.81
	2	3.977	br s (7.2)	79.90
	3	3.780	dd	9.7, 3.1	71.66
	4	3.440	dd	9.7, 9.6	73.71
	5	3.541	dq	9.6, 6.0	70.84
	6	1.273	d	6.0	18.51
α-l-Rhap 2	1	4.979	br s (4.5)		103.55
	2	4.264	br dd	3.1, 1.6	71.38
	3	3.900	dd	9.8, 3.1	81.13
	4	3.591	dd	9.8, 9.8	72.83
	5	3.756	dq	9.8, 6.1	71.04
	6	1.271	d	6.1	18.39
β-d-Quip4N (4-amino-4,6-dideoxy-β-d-glucopyranosyl)	1	4.726	d	8.0	105.20
	2	3.126	dd	9.2, 8.0	84.94
	3	3.517	dd	10.2, 9.2	74.50
	4	3.619	dd	10.2, 10.0	58.26
	5	3.535	dq	10.0, 6.0	72.56
	6	1.199	d	6.0	18.64
	2-O-Me	3.620	s	61.77
N-(3-hydroxy-1-oxobutyl)	1′	2.402	dd	14.1, 7.9	176.2c
	2′	2.422	dd	14.1, 5.5	46.87
	3′	4.188	ddq	7.9, 5.5, 6.3	66.73
	4′	1.218	d	6.3	23.77

^aObtained at 800.33 MHz for ¹H and 125.76 MHz for ¹³C in D₂O at 298 K. Numbers of rhamnosyl residues are based on the order from the reducing end.

^bs, singlet; d, doublet; t, triplet; q, quartet; br, broad; W_1/2, width at half height.

^cRead from a position of an HMBC cross peak.