TABLE 2.
Plasmidsa
| Name | Relevant characteristics | Reference |
|---|---|---|
| pJL72 (pEB793) | TAP-Kanr cassette; Ampr; ColE1 ori | 41 |
| pBAD24 (pEB227) | Ampr; pBR322 ori; PBAD promoter | 17 |
| pBAD24-entH (pEB921) | Ebm196/Ebm197 ybdB PCR ligated in pBAD24 using EcoRI and HindIII | This work |
| pBAD24-ydiI (pEB967) | Ebm146/Ebm147 ydiI PCR ligated in pBAD24 using EcoRI and HindIII | This work |
| pBAD24-CBP (pEB602) | Ampr; pBR322 ori; PBAD-CBP | 15 |
| pBAD24-CBP-entH (pEB847) | Ebm196/Ebm197 ybdB PCR ligated in pBAD24 using EcoRI and HindIII | This work |
| pBAD24-entH-TAP (pEB883) | Ebm196/Ebm223 ybdB PCR ligated with CtermTAP in pBAD24 | This work |
| pT25 (pEB354) | pKT25 derivative; Kanr; p15A ori; Plac promoter T25 domain | 15 |
| pT18 (pEB355) | pUT18C derivative; Ampr; ColE1 ori; Plac promoter; T18 domain | 15 |
| ybgC (pEB381-pEB380) | ybgC5′-ybgC3′ ybgC PCR in pT25 and pT18 using EcoRI and XhoI | 15 |
| yigI (pEB702-pEB715) | Ebm148/Ebm149 yigI PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| yciA (pEB710-pEB713) | Ebm144/Ebm145 yciA PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| ydiI (pEB654-pEB665) | Ebm146/Ebm147 ydiI PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| ybaW (pEB580-pEB579) | Ebm106/Ebm107 ybaW PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| fabA (pEB721-pEB720) | Ebm163/Ebm164 fabA PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| paaI (pEB831-pEB720) | Ebm198/Ebm199 paaI PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entH (pEB829-pEB830) | Ebm196/Ebm197 entH PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entA (pEB977-pEB974) | Ebm308/Ebm309 entA PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entB (pEB904-pEB902) | Ebm243/Ebm244 entB PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entB(S245A) (pEB922-pEB919) | Mutagenesis Ebm251/Ebm252 on pEB904 and pEB902 | This work |
| Flag-entB (pEB1018) | Hybridized Ebm363 and Ebm364 oligonucleotides inserted in pEB904 (PstI/XbaI) | This work |
| Flag-entB(S245A) (pEB1020) | Hybridized Ebm363 and Ebm364 oligonucleotides inserted in pEB922 (PstI/XbaI) | This work |
| entB ICL (pEB1002-pEB1000) | Ebm243/Ebm343 entB PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entB ArCP (pEB1003-pEB1001) | Ebm342/Ebm244 entB PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entE (pEB979-pEB976) | Ebm312/Ebm313 entE PCR in pT25 and pT18 using EcoRI and XhoI | This work |
| entC (pEB978-pEB975) | Ebm310/Ebm311 entC PCR in pT25 and pT18 using XbaI and XhoI | This work |
| entF (pEB916-pEB915) | Ebm250/Ebm262 entF PCR in pT25 and pT18 using PstI and XhoI | This work |
| acpP (pEB375-pEB379) | Ebm76/Ebm77 acpP PCR in pT25 and pT18 using EcoRI and XhoI | 15 |
a
Laboratory codes corresponding to our stock numbering are given in brackets. For the two-hybrid plasmids, both constructions in the pT25 and pT18 vectors are indicated on only one line per gene. Boldface type indicates the cloning vectors for each type of construct. ori, origin of replication.