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Detection of Ybt in planta. LC-MS analysis of Ybt from Arabidopsis leaf extracts harvested 5 dpi with P. syringae pv. tomato DC3000 (A), the pchA mutant (B), or control treatment (10 mM MgS04) (C). ESI (positive ion) of 200 to 1,200 for the elution time of Ybt-Fe3+ is shown. Major ion intensities (m/z) for the iron-bound Ybt monomer (m/z, 535.1; dimer, 1,068.7) are indicated with an arrow where present.
