FIG. 8.

Conditional experiments varying temperature, redox conditions, and pH show enhanced growth of the pchA mutant compared with Pst DC3000 under stringent iron-limiting conditions. Bacterial growth (OD₆₀₀) was assessed for cultures at 24 hpi under iron-limiting conditions (with 150 μM dipyridyl) with varying levels of temperature, oxidant (H₂O₂), reductant (DTT), or pH to assess differences in bacterial growth under potentially environmentally relevant conditions. Data are normalized to growth of DC3000 under standard conditions (20°C, pH 7.2).