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Localization of TagBΔN30GFP and TagBN30PhoA in B. subtilis 168. Whole-cell lysates (Lys.) of EB925 (tagBΔN30gfp) (A) and EB921 (tagBN30phoA) (B) were fractionated into soluble lysate (Sol.) and membrane samples (Mem.) by differential ultracentrifugation. Samples were subjected to immunodetection analysis using anti-GFP and anti-PhoA antisera, respectively. Samples were also probed with anti-TagD and anti-EzrA antisera to assess the quality of fractionation.
