FIG. 3.

In vivo functional analyses of TagBPhoA insertional variants. (A) TagB activity was monitored by complementation of the tag-1 allele at the restrictive temperature via xylose-inducible insertional variants of TagB. Strains EB1240 (empty vector; -ve), EB1652 (tagBphoA; wt), EB1653 (tagB+1phoA; +1), EB1654 (tagB+2phoA; +2), EB1655 (tagB+3phoA; +3), and EB1656 (tagB+4phoA; +4) were grown at the permissive temperature, diluted as indicated, spotted on LB-CHL-xylose solid medium, and grown at 47°C. (B) Immunodetection analysis of TagB variant expression from cell lysate using anti-PhoA antiserum. Immunodetection of FtsY levels was used as a loading control. The asterisk denotes a cross-reactive band.