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. 2007 Jul 27;189(19):6816–6823. doi: 10.1128/JB.00910-07

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Copyright © 2007, American Society for Microbiology

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FIG. 4. — Localization analyses of TagBPhoA insertional variants. (A) Strains EB1653 (tagB+1phoA; +1), EB1654 (tagB+2phoA; +2), EB1655 (tagB+3phoA; +3), and EB1656 (tagB+4phoA; +4) were grown under inducing conditions, harvested, and lysed. Whole-cell lysate (Lys.) was fractionated into soluble lysate (Sol.) and membrane samples (Mem.) by differential ultracentrifugation. Samples were subjected to immunodetection with anti-PhoA antiserum. Samples were also probed with anti-TagD and anti-EzrA antisera to assess the quality of the fractionation. The asterisk denotes a cross-reactive band. (B) Samples from strains EB1696 (tagBN30+1phoA; +1), EB1697 (tagBN30+2phoA; +2), EB1698 (tagBN30+3phoA; +3), and EB1699 (tagBN30+4phoA; +4) were fractionated as outlined above and subjected to the same immunodetection analyses.