FIG. 6.

Cleavage of nPP by vEP. vEP-34 (10 μg) was incubated without or with different amounts of nPP in a 30-μl reaction volume at 4°C (A) or 37°C (B) overnight. The final enzyme concentration was 9.8 μM. A 15-μl aliquot of each sample was then subjected to SDS-PAGE using a 12% gel (A and B), while 2-μl aliquots were diluted 100-fold into prewarmed (37°C) assay buffer containing azocasein and incubated for 10 min at 37°C to measure the residual protease activity (C). Data are means ± standard deviations from two experiments carried out in triplicate.