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. 2007 Jul 20;189(19):6832–6838. doi: 10.1128/JB.00396-07

TABLE 1.

Summary of the purification of vEP-45 and vEP-34

Fraction Total protein (mg)
Total activity (U)a
Sp act (U/mg)
Yield (%)
vEP-45 vEP-34 vEP-45 vEP-34 vEP-45 vEP-34 vEP-45 vEP-34
Periplasmic 75 118 282,000 545,000 3,750 4,620 100 100
Phenyl Sepharose HP 13 46 194,000 509,000 14,900 11,700 69 93
Superdex 75 100/300 GL 4.7 23 75,900 373,000 16,100 16,200 27 68
a

One unit is defined as the amount of enzyme that catalyzes the proteolysis of 1 μg of azocasein per min.