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Effect of the ganR mutation on ganE and ganK transcriptional fusionsa
| Fusion | Regulatory mutation | β-Glucuronidase sp actb |
|---|---|---|
| ganE::uidA | None | 9,374 |
| ganR | 1,158 | |
| ganK::uidA | None | 13,591 |
| ganR | 2,872 |
Bacteria were grown in glycerol minimal medium at 30°C. β-Glucuronidase activity was measured with PNPU as a substrate.
The specific activity is expressed as nanomoles of PNP liberated per minute per milligram of protein. The results reported are the average of three independent experiments.
