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EMSAs of the binding of H-NS and MBP-Hha to the rtsA promoter. The proteins were incubated with a 32P-labeled DNA fragment representing a portion of the rtsA promoter at positions −56 to +169. Samples were resolved by nondenaturing polyacrylamide gel electrophoresis, and the positions of the radioactive bands were detected by use of a PhosphorImager.
