FIG. 6.

DNase I protection assay of HilC, HilD, and MBP-RtsA binding to the rtsA promoter. The DNase I digestions represent the noncoding strand of the ³²P-labeled rtsA fragment from −173 to +29 incubated with increasing amounts of purified proteins. Protected bands are indicated on the left. Hypersensitive sites are shown by arrows. Lane 1 has the products of the A+G sequencing reaction of the same DNA fragment. The added proteins in each lane are as follows: lane 2, no added protein; lane 3, 150 nM HilC; lane 4, 300 nM HilC; lane 5, 150 nM HilD; lane 6, 300 nM HilD; lane 7, 150 nM MBP-RtsA; lane 8, 300 nM MBP-RtsA.