TABLE 1.
Bacterial strains, phages, and plasmids
| Strain, phage, or plasmid | Relevant characteristics | Reference and/or source |
|---|---|---|
| Bacterial strains | ||
| S. enterica serovar Typhimurium strains | ||
| CH1816 | hns-101::IS10 zde-1710::Tn10 Tetr | L. Csonka |
| IO907 | LT2 Δhha::cam hns-1::kan hilA080::Tn5 lacZY-080 Tetr Kanr Camr | 36 |
| S. enterica subsp. enterica ATCC 14028 derivatives | ||
| ATCC 14028 | WT | ATCC |
| JS324 | Φ(rtsA-lac+)5 Kanr | 14 |
| JS483 | ΔhilCD2915::cam Φ(rtsA-lac+)5 Kanr Camr | 13 |
| JS487 | Φ(hilC-lac+)113 Kanr | 13 |
| JS488 | Φ(hilD-lac+)114 Kanr | 13 |
| IO908 | hilA080::Tn5 lacZY-080 Tetr | This work |
| IO923 | Δhha::cam hns-1::kan hilA080::Tn5 lacZY-080 Tetr Kanr Camr | This work |
| IO949 | Δhha::cam Φ(rtsA-lac+)5 Camr Kanr | This work |
| IO954 | hns-101::IS10 zde-1710::Tn10 Φ(rtsA-lac+)5 Tetr Kanr | This work |
| IO955 | Δhha::cam hns-101::IS10 zde-1710::Tn10 Φ(rtsA-lac+)5 Tetr Kanr Camr | This work |
| IO959 | Δhha::cam Φ(hilD-lac+) Kanr Camr | This work |
| IO961 | hns-101::IS10 zde-1710::Tn10 Φ(hilD-lac+)Camr Kanr | This work |
| IO963 | Δhha::cam hns-101::IS10 zde-1710::Tn10 Φ(hilD-lac+) Tetr Kanr Camr | This work |
| IO969 | Δhha::cam Φ(hilC-lac+) Kanr Camr | This work |
| IO971 | hns-101::IS10 zde-1710::Tn10 Φ(hilC-lac+) Camr Kanr | This work |
| IO973 | Δhha::cam hns-101::IS10 zde-1710::Tn10 Φ(hilC-lac+) Tetr Kanr Camr | This work |
| IO976 | ΔhilCD2915 Φ(rtsA-lac+)5 Kanr | This work |
| IO997 | Δhha::cam ΔhilCD2915 Φ(rtsA-lac+)5 Kanr Camr | This work |
| IO999 | hns-101::IS10 zde-1710::Tn10 ΔhilCD2915 Φ(rtsA-lac+)5 Kanr Tetr | This work |
| IO1000 | Δhha::cam hns-101::IS10 zde-1710::Tn10 ΔhilCD2915 Φ(rtsA-lac+)5 Kanr Tetr Camr | This work |
| E. coli strains | ||
| DH5α | endA1 hsdR17 glnV44 thi-1 recA1 gyrA relA1 Δ(lac-argF)169 deoR [φ80dlacΔ(lacZ)M15] | Invitrogen |
| BL21(DE3) | ompT hsdSBgal dcm (λDE3) | Novagen |
| M182 hns | Δ(lacIPOZYA)74 galK strA Δhns-1::kan Kanr | M. Belfort |
| IO991 | BL21(DE3) Δhns-1::kan Kanr | This work |
| Phages | ||
| P22 HT | Laboratory stock | |
| P1 | Laboratory stock | |
| Plasmids | ||
| pRS415P | Ap; lacZYA transcriptional fusion plasmid | 37 |
| pRS415P derivatives | This work; 36 | |
| pMBPHis-parallel1 | Ap; expression vector | Z. Derewenda; 46 |
| pMBP-RtsA1 | Ap; MBP-His6-RtsA-expressing plasmid | This work |
| pHilC14 | Ap; His6-HilC-expressing plasmid | 37 |
| pHilD21 | Ap; His6-HilD-expressing plasmid | 37 |
| pHNS11 | Ap; MBP-His6-H-NS-expressing plasmid | 36 |
| pH3 | Ap; MBP-His6-Hha-expressing plasmid | 36 |