TABLE 1.
Primer | Sequence (5′→3′) | Amplicon size (bp) | Gene description |
---|---|---|---|
bll0322F | GGGGACGACCTGTGAACTTA | 251 | Trehalose 6-phosphate synthetase (otsA) |
bll0322R | CTTCGTAATAGCCGCCGTAA | ||
blr6767F | TTCTACTGGCACCGCTTCTT | 173 | Trehalose synthase (treS) |
blr6767R | GAGGTTCTCGTTCGAAGTGC | ||
blr6771F | CAAGCTGATCGACGATACCA | 218 | Maltooligosyltrehalose synthase (treY) |
blr6771R | AATTGGTAGAACGCGGTGTC | ||
blr0723F | CTTCGTGGAAGAGGAACTCG | 182 | rpoN2 |
blr0723R | CCTTGCTCATCCATTCTTCC | ||
blr1883F | ACGGCTTCTTTACCCTTGGT | 167 | rpoN1 |
blr1883R | GATCGATGCCGTGAAGAAAT | ||
bll0631F | GGACGCCGATTACACCTATG | 282 | Chromosome partitioning protein A, cppA (for normalization) |
bll0631R | GTAGACCTTCTCGCCCATGA |