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Spectrophotometric analysis of the conversion of 4-sulfomuconolactone by the purified sulfomuconolactone hydrolase from H. intermedia S1. The reaction mixture was contained in a final volume of 1 ml of 50 mM Tris-HCl (pH 8.0), 100 μM 4-sulfomuconolactone, and 5 μl of a purified preparation of the 4-sulfomuconolactone hydrolase from H. intermedia (Cprot = 0.5 mg/ml). The overlay spectra were recorded every min against a reference cuvette containing the same ingredients but no enzyme.
