TABLE 3.
Kinetic data for the conversion of 4-sulfomuconolactone by the 4-sulfomuconolactone hydrolases from H. intermedia S1 and A. radiobacter S2
| Enzyme | Source | Molecular mass per subunit (kDa) | Kinetic data ± SDa
|
|||
|---|---|---|---|---|---|---|
| Km (mM) | Vmax (U/mg) | kcat (min−1) | kcat/Km (mM−1 min−1) | |||
| HiSLH | H. intermedia S1 | 34.7 | 1.9 ± 0.5 | 79.0 ± 18.6 | 2700 ± 600 | 1400 |
| ArSLH | A. radiobacter S2 | 32.9 | 0.34 ± 0.14 | 12.6 ± 3.3 | 400 ± 100 | 1200 |
a
Kinetic data ± standard deviations (SD) were calculated by nonlinear regression using Prism 4 software (GraphPad Software, San Diego, CA). Vmax values were calculated based on the protein content.