TABLE 3.
Quantitative RT-PCR analysis of β-ketoadipate pathway genes
| Gene | Relative quantity of mRNA during growth witha:
|
Induction (n-fold) | |
|---|---|---|---|
| Succinate | Carbazole | ||
| catB (PP3715) | 0.0015 ± 0.0001 | 0.24 ± 0.02 | 160 |
| catA2 (PP3166) | 0.00021 ± 0.00002 | 0.0013 ± 0.0001 | 5.9 |
| pcaF (PP1377) | 0.00086 ± 0.00011 | 0.036 ± 0.005 | 41 |
| pcaD (PP1380) | 0.0015 ± 0.0001 | 0.014 ± 0.001 | 8.7 |
| pcaI (PP3951) | 0.0024 ± 0.0004 | 0.10 ± 0.01 | 42 |
| pcaJ (PP3952) | 0.0024 ± 0.0003 | 0.042 ± 0.004 | 18 |
a
cDNA was synthesized from total RNA of P. putida KT2440(pCAR1) during growth with succinate or carbazole by a standard method for microarray analysis. The mRNA level for each gene was divided by the 16S rRNA level. Values are averages ± standard deviations of results from at least five independent experiments.