TABLE 3.

Phenotype MicroArrray analysis of the MWX strain versus NCIB3610

Phenotype group and PM no.a	Well(s)	Growth conditions	Differenceb	Mode of actionc
Gained phenotypes (resistance)
PM32	A04	t-Butyl hydroquinone	43	Oxidizing agent
PM36	E09, E10	Neomycin	27	Protein synthesis, aminoglycoside
PM32	B01	Lincomycin	11	Protein synthesis, lincosamide
Lost phenotypes (sensitivity)
PM36	C02-C04	2-Hydroxybenzoic acid	−41	Anticapsule; multiple antibiotic resistance inducer
PM34	E10, E11	Warfarin	−55	Antimicrobial, from plants
PM31	B09-B12	Fusaric acid	−73	Chelator, lipophilic
PM31	C03	1-Hydroxy-pyridine-2-thione	−22	Chelator, lipophilic
PM35	C09	Cinoxacin	−41	DNA gyrase, DNA topoisomerase
PM35	A06	Miltefosine	−45	Fungicide, protein kinase C
PM36	A09-A11	Ibuprofen	−87	Inhibitor prostaglandin synthetase
PM31	E11	Chlorpromazine	−67	Inhibits cyclic nucleotide phosphodiesterase
PM36	A03	Alexidine	−79	Membrane, biguanide, electron transport
PM31	F05-F07	Lauroylsarcosine	−103	Membrane, detergent
PM31	H03, H04	Triton X-100	−137	Membrane, detergent
PM35	B04	CHAPSO	−50	Membrane, detergent
PM35	B08	Lauryl sulfobetaine	−24	Membrane, detergent
PM36	D11, D12	Dodecyl maltoside	−99	Membrane, detergent, nonionic
PM35	C04	Iodoacetate	−64	Oxidation, sulfhydryl
PM10	A03	pH 4.5	−38	pH
PM10	E08	pH 9.5 glycine	−23	pH, deaminase
PM10	F10	pH 9.5, l-ornithine	−26	pH, deaminase
PM10	F11	pH 9.5, l-homoarginine	−38	pH, deaminase
PM10	F12	pH 9.5, l-homoserine	−52	pH, deaminase
PM10	G02	pH 9.5, l-norleucine	−16	pH, deaminase
PM10	G03	pH 9.5, l-norvaline	−35	pH, deaminase
PM10	B03	pH 4.5, l-arginine	−49	pH, decarboxylase
PM10	B04	pH 4.5, l-asparagine	−32	pH, decarboxylase
PM10	B05	pH 4.5, l-aspartic acid	−35	pH, decarboxylase
PM10	B06	pH 4.5, l-glutamic acid	−86	pH, decarboxylase
PM10	B07	pH 4.5, l-glutamine	−74	pH, decarboxylase
PM10	B08	pH 4.5, glycine	−72	pH, decarboxylase
PM10	B09	pH 4.5, l-histidine	−71	pH, decarboxylase
PM10	B10	pH 4.5, l-isoleucine	−54	pH, decarboxylase
PM10	C02	pH 4.5, l-phenylalanine	−56	pH, decarboxylase
PM10	C04	pH 4.5, l-serine	−47	pH, decarboxylase
PM10	C05	pH 4.5, l-threonine	−57	pH, decarboxylase
PM10	C06	pH 4.5, l-tryptophan	−21	pH, decarboxylase
PM10	C07	pH 4.5, l-tyrosine	−43	pH, decarboxylase
PM10	C08	pH 4.5, l-valine	−59	pH, decarboxylase
PM10	D02	pH 4.5, l-norleucine	−34	pH, decarboxylase
PM10	D03	pH 4.5, l-norvaline	−82	pH, decarboxylase
PM10	D04	pH 4.5, a-amino-N-butyric acid	−23	pH, decarboxylase
PM10	D07	pH 4.5, g-hydroxy glutamate	−56	pH, decarboxylase
PM10	D11	pH 4.5, trimethylamine-N-oxide	−81	pH, decarboxylase
PM10	B01	pH 4.5	−61	pH, decarboxylase control
PM35	C06	Compound 48/80	−56	Phospholipase C, ADP ribosylation
PM32	D10-D12	Cadmium chloride	−35	Transport, toxic cation
PM32	E10-E12	Phosphomycin	−82	Wall
PM35	H07, H08	Glycine	−60	Wall
PM35	H12	Bacitracin, zinc salt	−99	Wall and membrane
PM32	G03, G04	Cefotaxime	−131	Wall, cephalosporin
PM32	G05	Cefazolin	−90	Wall, cephalosporin
PM32	H01	Cefoxitin	−58	Wall, cephalosporin
PM32	H05	Cefoperazone	−75	Wall, cephalosporin
PM33	F02-F04	Cefsulodin	−149	Wall, cephalosporin
PM33	F05, F06	Cephaloridine	−134	Wall, cephalosporin
PM33	F11, F12	Cefuroxime	−80	Wall, cephalosporin
PM33	G09, G10	Azlocillin	−107	Wall, β-lactam
PM32	H09, H10	d-Cycloserine	−100	Wall, sphingolipid synthesis

^aPhenotype MicroArrray (PM) plate numbers are as described on the Biolog website (see Materials and Methods).

^bGrowth measurements were done using turbidity as described in Methods and Materials. Negative values indicate significantly poorer growth of the MWX strain relative to NCIB3610 while positive values indicate better growth of the MWX strain relative to NCIB3610.

^cPossible effect or mode of action (original Biolog annotation). Not all modes of action are applicable to B. subtilis.