TABLE 2.
Characteristics of suspected H. cinaedi isolates and other, related organisms
| Isolate no. | Institution | Growth at 25°C/42°Ca | Growth on CCDA agara,b | Suspected identification by culture results | 16S rRNA sequence analysis
|
||
|---|---|---|---|---|---|---|---|
| % Similarity (primer) | GenBank accession no. | Identification | |||||
| 4 | Toho University Omori Medical Center | +/+ | + | C. fetus | 98 (middleF) | AF550619.1 | C. fetus subsp. fetus |
| 5 | Toranomon Hospital | −/+ | − | H. cinaedi | 98 (middleF) | M88150.2 | H. cinaedi |
| 9 | Toho University Omori Medical Center | −/+ | − | H. cinaedi | 99 (middleF) | M88150.2 | H. cinaedi |
| 11 | Toranomon Hospital | −/+ | − | Helicobacter sp. | 98 (middleF) | M88150.2 | H. cinaedi |
| 12 | Nihon University Itabashi Hospital | −/− | − | H. cinaedi | 99 (8F + middleF) | HECRR16SAE | H. cinaedi |
| 13 | Toho University Omori Medical Center | +/+ | + | C. fetus | 98 (middleF) | AF550619.1 | C. fetus subsp fetus |
| 14 | Nihon University Itabashi Hospital | −/− | − | Helicobacter sp. | 99 (8F+middleF) | HECRR16SAE | H. cinaedi |
| 15 | Surugadai Nihon University Hospital | +/− | + | C. fetus | 99 (middleF) | AF550619.1 | C. fetus subsp fetus |
| 16 | Toranomon Hospital | −/− | − | Helicobacter sp. | 98 (middleF) | HECRR16SAE | H. cinaedi |
a
+, growth; −, no growth.
b
Charcoal cefoperazone deoxycholate agar (CCDA) was a specially designed medium for the isolation of Campylobacter spp., and isolates were incubated microaerobically at 42°C for 40 to 48 h.