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Linear dynamic range of the real-time PCR assays 8653 (A) and 9438 (B). The assays were performed with triplicates of 10-fold dilutions of the purified genomic DNA extracted from B. pseudomallei strain K96243. The regression line representing the linear range of 106 to 102 fg per PCR was obtained using LightCycler software (Roche Molecular Biochemical, version 3.5) for both assays (A and B), respectively.
