TABLE 3.
Evaluation of the real-time PCR assays with DNA extracted from blood specimensa
| Clinical diagnosis and blood specimen | Patient information
|
Mean CT value (no. of CT values used) for:
|
|||
|---|---|---|---|---|---|
| Clinical outcome | Type of positive culture | Assay 8653 | Assay 9438 | Assay for orf11 | |
| Septicemia by Burkholderia pseudomallei | |||||
| BP1 | Survived | B | 32.23 (5) | 32.56 (2) | 30.21 (2) |
| BP2 | Survived | B | 32.68 (5) | 34.28 (7) | 32.13 (2) |
| BP3 | Survived | B/P/TS | 33.80 (5) | 33.54 (6) | 31.28 (2) |
| BP4 | Died | B/TS/U | 30.87 (5) | 33.73 (4) | 33.53 (2) |
| BP5 | Died | B/S/TS | 36.03 (6) | 37.48 (4) | 37.35 (2) |
| BP6 | Survived | B/P | 32.92 (3) | 34.16 (7) | NA |
| BP7 | Died | B/P/S/TS/U | 25.32 (5) | 24.35 (6) | 22.71 (2) |
| BP8 | Survived | P | 34.60 (4) | 38.74 (4) | Neg |
| BP9 | Survived | P | 34.77 (3) | 36.77 (3) | 38.23 (1) |
| BP10 | Died | B/P/S/U | 32.50 (5) | 34.06 (4) | NA |
| BP11 | Survived | B/P/S/U | 33.49 (4) | 37.48 (4) | Neg |
| BP12 | Survived | B/P | 34.64 (5) | 36.90 (7) | 37.07 (2) |
| BP13 | Survived | P/TS | 37.80 (5) | 36.63 (2) | 39.12 (2) |
| BP14 | Died | B | 32.30 (9) | 33.67 (6) | 35.10 (2) |
| BP15 | Survived | B | 35.59 (6) | 34.61 (2) | 42.27 (1) |
| BP16 | Survived | B | 33.64 (2) | Neg | NA |
| BP17 | Survived | B | 33.66 (3) | Neg | 32.96 (2) |
| BP18 | Survived | P | 35.41 (3) | Neg | Neg |
| BP19 | Died | B/S | 34.62 (9) | Neg | 37.53 (2) |
| BP20 | Died | B/TS | 33.02 (2) | Neg | Neg |
| BP21 | Survived | B | Neg | Neg | Neg |
| BP22 | Survived | B/TS | Neg | Neg | Neg |
| BP23 | Survived | P | Neg | Neg | Neg |
| BP24 | Survived | S/TS | Neg | Neg | Neg |
| BP25 | Survived | B/P | Neg | Neg | Neg |
| BP26 | Survived | B | Neg | Neg | Neg |
| BP27 | Survived | P | Neg | Neg | Neg |
| BP28 | Survived | B/P/U | Neg | Neg | Neg |
| Septicemia by Burkholderia cepacia | |||||
| CI1 | Died | B/S | Neg | Neg | Neg |
| Septicemia by Escherichia coli | |||||
| CI2 | Survived | B | Neg | Neg | Neg |
| CI3 | Survived | B | Neg | Neg | Neg |
| CI4 | Survived | B/U | Neg | Neg | Neg |
| CI5 | Survived | B | 33.56 (6) | 33.83 (5) | 39.31 (2) |
| Septicemia by Enterobacter sp. | |||||
| CI6 | Survived | B | Neg | Neg | Neg |
| Septicemia by Klebsiella pneumoniae | |||||
| CI7 | Survived | B | 29.92 (4) | 30.03 (6) | 33.3 (2) |
| CI8 | Died | B/S | Neg | Neg | Neg |
| CI9 | Survived | B/S/U | Neg | Neg | Neg |
| CI10 | Survived | B | Neg | Neg | Neg |
| CI11 | Survived | B | Neg | Neg | Neg |
| Septicemia by Staphylococcus aureus | |||||
| CI12 | Died | B/P/S | Neg | Neg | Neg |
| CI13 | Survived | B | 36.27 (5) | Neg | Neg |
| CI14 | Survived | B/U | Neg | Neg | Neg |
| Septicemia by Streptococcus sp. | |||||
| CI15 | Died | B | Neg | Neg | Neg |
| CI16 | Survived | B/CSF | Neg | Neg | Neg |
| CI17 | Died | B | Neg | Neg | Neg |
a
Evaluation of the real-time PCR assays was performed with DNA extracted from blood specimens collected from 45 septicemic patients admitted to various hospitals in the northeastern region, Thailand. Abbreviations: B, blood; P, pus; S, sputum; TS, throat swab; U, urine culture; CSF, cerebrospinal fluid culture; Neg, negative amplifications; NA, not enough DNA samples were left for performing these real-time PCRs.