TABLE 2.
Primers for the construction of the expression vectorsa
| Designation | Position (nt) | Sequenceb | Polarity |
|---|---|---|---|
| SP1 | 160-176 | 5′-ACGGAATTCGAGAACATCGCATCAGG-3′ | Sense |
| SP2 | 824-841 | 5′-GCACTGCAGCGGTTTAAATGTATACCC-3′ | Antisense |
| P120TL | 494-527 | 5′-CCTCAACAACCAGCACGGGAACATGCAAGACC-3′ | Sense |
| C121SL | 494-531 | 5′-CCTCAACAACCAGCACGGGACCAAGCAAGACCTGCA-3′ | Sense |
| K122IL | 494-537 | 5′-CCTCAACAACCAGCACGGGACCATGCATCACCTGCACGATTCC-3′ | Sense |
| T123NL | 494-537 | 5′-CCTCAACAACCAGCACGGGACCATGCAAGAACTGCACGATTCC-3′ | Sense |
| T-primer | 490-513 | 5′-CCCGTGCTGGTTGTTGAGGATCC-3′ | Antisense |
| MP-145-1 | 578-599 | 5′-AACCTACGGACAGAAACTGCAC-3′ | Sense |
| MP-145-2 | 575-598 | 5′-TGCAGTTTCTGTCCGTAGGTTTTG-3′ | Antisense |
a
The primers were designed according to the sequence of the HBV genome with accession number AF100308.
b
The added sequences containing the restriction sites in SP1 and SP2 are in boldface. The restriction sites are underlined. The mutated codons in primers P120TL, C121SL, K122IL, and T123NL are italicized.