FIG. 2.

Representative results from IFA protocol and replicon protocol. (A) Vero cells infected with the supernatant from the first passage of clinical specimens were fixed at 3 days postinfection. The presence of virus was determined by the detection of viral structural proteins using rubella virus-specific mouse monoclonal antibodies and AlexaFluor 488-conjugated secondary antibody (green). The nuclei were stained using propidium iodide (red) as a counterstain. (B) GFP expressed from replicons was detected in Vero cells only when the infectious virus was present in the specimen (RVTS20) but not in the absence of infectious virus (RVTS35).