FIG. 4.

Genetic relationships among 88 V. vulnificus isolates based on neighbor-joining cluster analysis of SSR variation. A set of 56 new environmental isolates were analyzed, together with the panel of 32 strains (Table 1). A genetic-distance matrix was generated based on 168 polymorphic points of 12 SSR loci. Biotype 1, circles; biotype 2, squares; biotype 3, inverted triangles; unidentified biotype, diamond; new environmental isolates, triangles. Underlined isolates: VVyb1, biotype 3; VVyb2 through VVyb10, biotype 1.