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Virion GP of EBOV consists of two cleavage subunits. EBOV GP metabolically labeled with [3H]glucosamine or with [35S]methionine-cysteine was immunoprecipitated from purified virions grown in Vero E6 cells and analyzed by 15% SDS/PAGE under reducing or nonreducing conditions. The positions of the mature GP (GP1,2), and the two cleavage subunits GP1 and GP2, are indicated.
