Figure 6.

Processing of wild-type and cleavage site mutants of EBOV subtype Reston. HeLa cells were infected with vTF7-3 and transfected with the plasmids pGEM-PR8 (wild-type Reston GP, WT), pGEM-R/K (mutant R/K), and pGEM-R/R (mutant R/R). At 6 h postinfection, cells were pulse-labeled for 20 min and chased for 240 min. Immunoprecipitated proteins were separated under reducing conditions on an 8% polyacrylamide gel. The positions of the noncleaved precursors (preGP_er and preGP) and the cleavage subunit GP₁ are indicated. The sequences at the cleavage sites are shown at Top.