TABLE 1.

Species specificity of the LiPA for detecting M. tuberculosis pncA

Species	Straina	Nested PCR resultb	Hybridization signal with probes:
			1-24	25-47
M. tuberculosis	H37Rv (ATCC 27294)	+	All positive	All positive
M. tuberculosis	H37Ra (ATCC 25177)	+	All positive	All positive
M. bovis	BCG Japanese strain 172c	+	Δ16d	All positive
M. avium	ATCC 25291	−*	All negative	All negative
M. fortuitum	RIMD 1317004 (ATCC 6841)	−*	All negative	All negative
M. gastri	GTC 610 (ATCC 15754)	−*	All negative	All negative
M. intracellulare	JCM 6384 (ATCC 13950)	−*	All negative	All negative
M. kansasii	JCM 6379 (ATCC 124878)	−	All negative	All negative
M. marinum	GTC 616 (ATCC 927)	−*	All negative	All negative
M. nonchromogenicum	JCM 6364 (ATCC 19530)	−*	All negative	All negative
M. phlei	RIMD 1326001 (ATCC 19249)	−	All negative	All negative
M. scrofulaceum	JCM 6381 (ATCC 19981)	−	All negative	All negative
M. simiae	GTC 620 (ATCC 25275)	−	All negative	All negative
M. smegmatis	ATCC 19420	−	All negative	All negative
M. szulgai	JCM 6383 (ATCC 35799)	−	All negative	All negative
M. terrae	GTC 623 (ATCC 15755)	−	All negative	All negative
Escherichia coli	ATCC 8739	−	All negative	All negative
Haemophilus influenzae	IID 984 (ATCC 9334)	−	All negative	All negative
Klebsiella pneumoniae	IID5209 (ATCC 15380)	−	All negative	All negative
Legionella pneumophila	GTC 745	−	All negative	All negative
Mycoplasma pneumoniae	IID 817	−	All negative	All negative
Pseudomonas aeruginosa	ATCC 27853	−	All negative	All negative
Rhodococcus equi	ATCC 33710	−	All negative	All negative
Staphylococcus aureus	N315	−	All negative	All negative
Streptococcus pneumoniae	GTC 261	−	All negative	All negative

^aATCC, American Type Culture Collection, Manassas, VA; RIMD, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan; GTC, Gifu Type Culture Collection, Department of Microbiology-Bioinformatics, Regeneration and Advanced Medical Science, Gifu University, Graduate School of Medicine, Bacterial Genetic Resources, Gifu, Japan; JCM, Japan Collection of Microorganisms, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan; IID, Institute of Medical Science, University of Tokyo, Tokyo, Japan.

^bApproximately 100 ng of genomic DNA was used in the first PCR. Amplification results were determined by agarose gel electrophoresis. +, presence of amplification product of the expected; −, absence of amplification products; −*, presence of amplification products, but the sizes of the products were different from that of M. tuberculosis.

^cFrom Japan BCG Laboratory, Tokyo, Japan.

^dAbsence of hybridization signal with one of the probes (probe 16).