FIG. 1.

Effects of hybridization temperature (A), time (B), streptavidin-R-phycoerythrin (SA-PE) concentrations (C), and wash conditions (D) on hybridization of C. hominis (hatched bars) and C. parvum (white bars) capture probes to ML-2 amplicons. The bars represent means and standard deviation values of the median fluorescence intensity (MFI) from two and more experiments, each with duplicate data points. Equal volumes of PCR products were used to determine the optimal conditions for hybridization. Panels A, B, C, and D demonstrate that maximal MFI values were obtained with a hybridization temperature of 42°C, hybridization time of 50 min, SA-PE at a dilution of 1:40, and when samples were washed, respectively.