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RFLP-specific band patterns of the capsular loci of serotypes Ia, Ib, II to V, and VII. A fragment encompassing the cpsG-neuA region was digested simultaneously with MspI and XbaI. “1Kb Plus” denotes the 1-kb Plus DNA ladder (Invitrogen, Carlsbad, CA). Numbers on the left indicate the sizes of the fragments. The rightmost lane is a different pattern presented by a single isolate of serotype II that is compatible with a missing recognition sequence of the MspI endonuclease.
