TABLE 1.
Characteristics of the putative products of the ORFs associated with S. agalactiae serotype II
| Putative protein | Highest-scoring homologue (GenBank accession no.)a | E-value (TBLASTX) | Organism (serotype)b | Proposed function |
|---|---|---|---|---|
| Cps2E | CpsE (AAL56285) | 0 | S. agalactiae (III) | Glycosyl transferase |
| Cps2F | CpsF (NP_735687) | 8 × 10−83 | S. agalactiae (III) | β-1,4-Galactosyl transferase enhancer |
| Cps2G | CpsG (AAK43608) | 2 × 10−89 | S. agalactiae (IV) | β-1,4-Galactosyl transferase |
| Cps2H | CpsH (NP_688177) | 7 × 10−47 | S. agalactiae (V) | CPS polymerase |
| Cps2Ic | CpsI (AAF18944) | 2 × 10−72 | Streptococcus suis | β-1,3-N-acetylglucosaminyltransferase |
| Cps2J | CpsJ (AAK43612) | 1 × 10−34 | S. agalactiae (IV) | Putative glycosyl transferase |
| Cps2P | BT_2870 (NP_811782) | 4 × 10−21 | Bacteroides thetaiotaomicron | Putative glycosyl transferase |
| Cps2Q | Eps3O (AAL23738) | 3 × 10−29 | Streptococcus thermophilus | Putative glycosyl transferase |
| Cps2K | CpsK (AAK11668) | 2 × 10−52 | S. agalactiae (VI) | α-2,3-N-Acetylneuraminyl transferase |
| Cps2L | CpsL (AAK11669) | 0 | S. agalactiae (VI) | CPS repeat unit transporter |
| NeuB | NeuB (NP_688170) | 0 | S. agalactiae (V) | N-Acetylneuraminic acid synthetase |
| NeuC | NeuC (AAR25957) | 0 | S. agalactiae (VII) | N-Acetylglucosamine-2-epimerase |
| NeuD | NeuD (NP_688168) | 1 × 10−116 | S. agalactiae (V) | Putative acetyltransferase |
| NeuAd | NeuA (AAK43618) | 6 × 10−62 | S. agalactiae (IV) | CMP-N-acetylneuraminic acid synthetase |
a
Serotype II sequences were excluded from the analysis due to the inconsistencies described in the text.
b
Whenever the highest-scoring homologue was an S. agalactiae protein, the serotype of the strain is also shown.
c
Although the BLAST highest-scoring homologue is an S. suis protein, this putative protein groups with a high bootstrap value with the CpsI proteins from other GBS serotypes.
d
The region sequenced does not include the complete gene.