TABLE 1.
Detection of Shiga toxins in fresh stool specimens and corresponding broth enrichment cultures by prospective screening with the BioStar OIA SHIGATOX and Premier EHEC kitsa
| Test | No. of results
|
Sensitivity (%) | Specificity (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| True positive | True negative | False positive | False negative | |||||||||
| Direct stool | ||||||||||||
| OIA | 30 | 707 | 4 | 1 | 96.8b | 99.4 | ||||||
| EIA | 26 | 710 | 1 | 5 | 83.9b | 99.8 | ||||||
| Broth enrichment | ||||||||||||
| OIA | 31 | 711 | 0 | 0 | 100 | 100 | ||||||
| EIA | 30 | 711 | 0 | 1 | 96.8 | 100 | ||||||
a
A total of 742 specimens were tested. Positive reactions were confirmed by a Vero cytotoxicity assay.
b
Significantly different from the result by the other method (P < 0.05).