TABLE 1.

Summary of MAC-ELISA results for 134 sera tested with WT and mutant SLEV and WNV antigens^a

Disease state	DRL diagnostic serum classification					This study's diagnostic serum classification
	No. of sera	No. of IgM-positive sera				No. of sera	No. of IgM-positive sera
		SLEV		WNV			SLEV		WNV
		WT	DRR	WT	RH		WT	DRR	WT	RH
SLEV	38	34	36	17	9	37	35	37	17	9
WNV	56	27	10	53	53	55	28	11	55	55
Non-SLEV/WNV flavivirus (JEV, DENV, POWV):	10	0	0	4	0	10	0	0	4	0
JEV	4	0	0	2	0	4	0	0	2	0
DENV	4	0	0	2	0	4	0	0	2	0
POWV	2	0	0	0	0	2	0	0	0	0
Flavivirus	16	4	2	4	2	3	2	0	2	0
Unknown flavivirus	8	4	2	4	2	3	2	0	2	0
Previous SLEV, WNV, or flavivirus	8	0	0	0	0	NAb
Nonflavivirus (WEEV, EEEV, LACV)	12	0	0	0	0	12	0	0	0	0
Unknown	2	0	0	0	0	17	0	0	0	0
Total	134	65	48	78	64	134	65	48	78	64

^aAll 134 sera were tested in this study with WT and mutant VLP antigens for both WNV and SLEV. Results are grouped here and for further analyses according to disease states determined either (i) previously by the DRL or (ii) by using this study's diagnostic classification. As described in detail in Materials and Methods, DRL disease state classifications are based upon MAC- or antigen-capture IgG-ELISA results using WT SLEV and WNV antigens only and PRNT data. This study's diagnostic classifications were based solely on MAC-ELISA results using WT and mutant VLP antigens without prior knowledge of the DRL classifications. The different numbers of WNV- and SLEV-infected sera classified by DRL and in this study are explained in detail in Materials and Methods and in Table 6.

^bNA, not applicable.