TABLE 3.
Influence of WT and mutant antigens on performance of MAC-ELISA in distinguishing the infected serum panel from other arbovirus-infected serum panelsa
| Target panel | Control panels | Antigen | AUC (95% CI) | Critical ratio (z) | Discriminatory probability (95% CI)
|
|
|---|---|---|---|---|---|---|
| % Sensitivity | % Specificity | |||||
| WNV infections | SLEV and remaining groups | WNV-WT VLP | 0.97 (0.95-1.00) | 1.34 | 100.0 (93.5-100.0) | 70.9 (59.6-80.6) |
| WNV-RH VLP | 0.99 (0.97-1.00) | 100.0 (93.5-100.0) | 88.6 (79.5-94.7) | |||
| SLEV infections | WNV and remaining groups | SLEV-WT VLP | 0.94 (0.90-0.98) | 2.18 | 94.6 (81.8-99.3) | 69.1 (58.9-78.1) |
| SLEV-DRR VLP | 0.98 (0.96-1.00) | 100.0 (90.5-100.0) | 88.7 (80.6-94.2) | |||
a
Shown is the influence of WT and mutant antigens on performance of MAC-ELISA in distinguishing the infected serum panel (target panel) from other arbovirus-infected serum panels (control panels) using a P/N ratio of ≥3 as the cutoff for the presence of antigen-specific IgM. All statistical analyses are based upon the panels as defined by our disease state classification.